Greetings,
I hope everyone is doing well out there. I need your guidance related to seaweed's extraction. I need to check few activities of seaweeds such as antioxidant, anti-inflammatory, anti-obesity and others for my research. Kindly do guide me in this regard so that I will get the best way to do this. I am looking forward to your positive response.
Thank you in anticipation.
Be Blessed.
Chapter 9 - Extraction of biomolecules from seaweeds
Seaweed Sustainability Food and Non-Food Applications2015, Pages 243-269
Chapter Extraction of biomolecules from seaweeds
Collect the seaweeds in any particular season (winter or summer or rainy) only once required for your entire experimental study. Wash it thoroughly with tape water thrice, remove the fouling organisms attached to the seaweed, then wash thoroughly with distilled water, dry it in shadow (while drying care must be taken to watch daily to avoid fungal contamination), shuffle it daily. Chopped it well. Weigh it. Put it in a air tight glass container. Add the solvent (preferably highly polar solvent-pure). Don't use water in any cause. Keep it for 7 - 10 days. Shake it well daily for complete extraction. After that, filter the solvent by using muslin cloth. Dry the biomass. Weigh it. Keep the filtrate in a lyophiliser for drying. Weight it after drying. Calculate the percentage of extraction= Weight of the plant material taken for extraction-weight of the plant material after extractionx100.
1- collect ( describe season and place)
2- wash with tap water
3- dry by hunging at shade
4- grind by mill
5- extract , start by nonpolar solvent, n-hexan, ethyl acetate, methanol and 70%methanol :water
6- take TLC for extracte
7- evaporate the solvents
8- check the biological activities for all extracts sepately
Wash and shade dry the samples after collection. Then grind the material and make extract by using 80%methanol in cold infusion method and also maintain the sample and solvent ratio at 1:100
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