thank you about STZ-icv , but what about cholinergic model or amyloid beta model ? i don't know which model is more correlate with human alzheimer pathology

I don't know exactly what you mean by "cholinergic model", but if you want to test the memory enhancing effect of a compound, you can use pharmacological transient amnesia models like the scopolamine model. Scopolamine is a muscarinic antagonist that causes transient memory impairment when administered systematically. So you can test whether a compound can reverse this impairment.

This is a commonly used model in behavioural pharmacology, however as you can see, this is not a real dementia-model as it does not mimic structural changes (amyloid, tau, neuronal loss) and neurodegeneration. For a reference, see our recent publication (linked, I can send you the full text if you request it).

Another cholinergic model can be the selective lesion of the cholinergic afferents to hippocampus, but I've never performed it.

Actually, there are no best (or even good) animal models of dementia/AD, you may need to try different models. It depends also on your aims (i.e, do you study behaviour, memory, gene expression etc.).

Article Differential effects of α7 nicotinic receptor agonist PHA-54...

Hi!  A nontransgenic AD model would be extremely desirable for the research community but unfortunately will never be available. You may certainly imitate certain isolated aspects of the disease pathophysiology, but not AD in its whole complexity. To induce tau pathology you may consider a phosphatase inhibitor like okadaic acid. However, conclusions from an animal model (or in vitro models) on human AD should be performed with utmost care.

actually i want to have proteomic study on non-transgenic rat model in alzheimer's disease and see if there any correlate between proteome hippocampus in rat model of AD and human with AD.

what i meant about cholinergic model was to make a deficit or lesion in cholinergic system and see proteome consequence.

In the review of Hasselmo (2006) about acetylcholine and memory (see the link), you may find references about the cholinergic lesion model.

http://www.sciencedirect.com/science/article/pii/S095943880600122X#

thank all of you for helping 

If you're looking at a non-transgenic rat model of AD, have a look at the following publication (see end of message). As someone previously explained, modeling all aspects of the human pathology is complex and difficult, but this model incudes a wide range of AD-like symptoms (behavioral and molecular). Have a look at other publications from this group for more information.

Hope this helps.

Article Time-Course and Regional Analyses of the Physiopathological ...