I am going to measure the leaf water content from coal char and biochar treated soils to see the soil moisture content change in soil due to char application and its reflection in plant leaf water content compared to control.
Pressure Bomb apparatus by LiCor produces very good and reproducible results ..time of sampling has to be standardized
Dear Dr. Anoop, it depends on what you want to know: if water activity or total water contents. There are different techniques for either one. So, indicate what is your intent with measuring water contents in leaves.
Dr Renar, we used it for leaf water content as a function of leaf water potential , especially at pre- dawn leaf water potential.....any further doubt , I am ready to furnish ....
They are many instuments are their to measure leaf water content and leaf water potential especially Infrared gas analyser (IRGA). Manually also one can measure leaf water content using following formula. Relative water content (RWC) of leaves =(Fresh weight -dry weight)/(turgid fresh weight-turgid dry weight). You can find many literature on RWC
We can also measure relative leaf water content (RLWC) and conopy temperature. For enumeration of water content in leaf, first we have to take fresh weight of leaf subsequently dry weight of leaf after keeping the sample in over dryer for 105°C temperature for 48-72 hours .. simultaneously we have to record the turgid weight of the sample...after that put the values in the formula to get relative water content in per cent..formula may be used is:
(FW-DW/TW-DW)X 100
Also for measuring canopy temperature, one can use canopy thermometer..for this, separately measurement of air temperature and canopy temperature, record the difference and then conclude if TC>Ta means there is water stress or if the Tc-Ta value is negative, means less water content..
Thank you, Dr. Srivastava, Dr. Renar, Dr. Rajanna, and Dr. Verma! for your valuable suggestions. I appreciate your quick response to my query. It's a lot for me to understand the leaf water measurement mechanism.
Resham
Terahertz spectroscopic techniques are particularly promising as a non-invasive tool for measuring leaf water content.
Cheers,
Ankur
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