What are the best methods for isolation of plasma membrane (PM) proteins from mammalian cells?

24 July 2018 1 2K Report

1. I need PM proteins as much as I can get but have to a void other interfering proteins, say from cytosolic, mitochondria, and organelle membrane. Can anyone suggest me the efficient, easy and reproducible methods to get PM?

2. It is nessecessary to show the WB when I have reliable MS data?

Milan Teraiya

Thanks Andrea!!

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