what are the best available databases for alternatively spliced isoforms in humans? How ESTs are used for identification of alternative spliced forms?
You can use UCSC Genome browser to view all the splice variants of a particular gene. There are several other databases also for finding isoforms. The following article might answer your question.
https://genome.ucsc.edu/cgi-bin/hgGateway
http://projects.insilico.us/SpliceMiner/intro.jsp
http://genome.cshlp.org/content/14/5/976.full
Hi
try to look at UniProt, used by SpliceSeq to analyse alternative splicing in RNAseq studies.
fred
Yes, UCSC genome browser list all splice forms that have been observed. Similarly the ENSEMBL genome browser form the EMBL also lists splice variants. These are from EST that have been sequenced from a variety of sources, so it is not always possible to tell from which tissue or cell type the splice form is coming form or how frequently that splice form is observed. These genome browsers merely list all possible forms that have been sequenced by the various EST projects over the last 20 years or so. .
of course you can use the browser from UCSC, but for a database prefer to go to the table browser from the same engine. the table will give you all what you can see in the viewer.
fred
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