What are the best tools and study protocol to study the beneficial soil microorganism and the symbiotic agents?

I am going to conduct the field survey to collect the major soil living organisms, beneficial insects, natural enemies, and decomposers and symbiotic agents. For each study, I am looking for...

28 July 2020 8,298 3 View

What could be the best way of staining our DNA during gel electrophoresis using Gel Red? Post staining or pre-staining and what concentration?

We use gel red in our lab to stain our DNA for gel electrophoresis. The bands obtained are wavy and smeary sometimes. How shall we overcome this problem?

20 February 2020 3,302 3 View

Can grounded theory be used whilst utilizing socio-ecological model as theoretical lens with probing for interview guide approach?

My research is a pure qualitative research and I am unable to find any literature answering my research question through socio-ecological model, thus I thought of grounded-theory with...

25 April 2019 1,459 8 View

How can I measure and optimize pH dependence of an EC Biosensor measurement?

Can I use NaOH solution to increase the pH of the system to a desired level ? Does NaOH have effect on the sensing property of the electrode ? If I can how can I do it ? If not how would I do it ?

05 January 2016 8,537 8 View

How can we clean the MIP electrode after measuring each concentration of analyte for calibration curve?

I am using Screen Printed Carbon Electrodes to fabricate Molecularly Imprinted Polypyrrole based sensor for tetracycline detection.

19 November 2015 1,560 2 View

Rab-YFP can not be recognized by Anti-YFP antibody but works with Anti-GFP antibody?

Recently we ordered YFP- tagged Rab protein (Rab-YFP) drosophila fly lines (for 15 different Rab proteins) from VDRC. Unfortunately none of them can be recognized by YFP antibody, but can be...

09 September 2014 3,341 1 View

Why are variations obvious in images but not in MTT assay readings?

During the MTT assay with h357 cell line using different types of culture plates ie ELISA, PLAIN and normal culture plate, I found that when observed under microscope there was a considerable...

22 August 2014 3,019 5 View

Are there any databases available for different types of clouds?

I am working on cloud discrimination against various earth's surface and hence need databases of different types of clouds.

01 January 1970 5,261 7 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View