What are the available good RACE kit ?

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Easily transfectable colorectal cancer cell lines like SW480 ?

Kindly suggest some colorectal cancer cell lines which can be easily transfectable..

01 February 2016 7,161 8 View

Does anyone know about easily transformable colorectal cancer cell lines?

Which colorectal cancer cell lines are easily transformable using lipofectamine apart from HCT-116.

06 July 2015 7,082 3 View

How to decide between rank and quantile normalization for gene expression microarray data normalization?

11 December 2014 1,207 3 View

What does "offset" and "quantile normalization" means in LIMMA ?

In LIMMA for gene expression data normalization, offset is used to correct background and quantile for between array normalization. How same works and setting different offset values like 16 or...

11 December 2014 725 5 View

What is the status of CTNNB1 and/or APC gene and microsatellites (MSS or MSI) in following cell lines, TTITKB, JHCOLOYI, JHSK-rec, HRA-16 and HRT-18?

I want to study non canonical pathway in colorectal cancer.

10 November 2013 212 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

Ajay Saini

Hi Raju,

The RACE kits from Invitrogen work well

bye

Raju Kumar

thanks

Michelle Wu

mRNA Decapping enzymes and Tabasco decapping enzymes are available to replace the TAP used in 5'RACE. Here is the link for details:

http://www.enzymax.net/mRNA_decapping_enzymes.htm

Anoumid Vaziri

This might be a really late answer, but I have been using Ambion RLM-RACE with success for a while now!