What is the best machine learning method for determine shock in time series?

I'm familiar with the Auto Vector Autorgressive (VAR) models. As you know, these models are widely used in determining shocks in time series. Also, machine learning methods have become very...

29 August 2020 9,668 5 View

What are algorithms that can be used for fined grained image classification?

I'm working on training a model that classify models of vehicles so i want to know which architectures should i use

24 March 2020 996 5 View

How can i calculate height and width of images for Object detection model yolo training ?

for my object detection model yolo i have annotation xml file with the bounding box coordinates (ymin, xmin, ymax, xmax) but don't have height and width information how can i calculate them or if...

18 March 2020 7,826 4 View

What is the best predicitve model and algorithms for predicting next customer's purchase ?

i have a dataset that contains customer_id transactions products and category . the result that i found when i apply the apriori algorithm and association rules was not good so could you please...

10 July 2019 1,076 3 View

How can i find a pump dataset for predictive maintenance ?

i am working on predictive maintenance of a pump but i didn't find a dataset.

22 January 2019 7,969 6 View

Calculating the intra and inter assay variation?

I performed a PCR on 25 samples in order to find a difference in the melting points by the mely curve analysis. I have divided my samples into three groups: homozygotes, heterezygotes and...

29 February 2016 9,572 2 View

Can someone explain me how a taqman actually works? What is the difference with a normale qPCR?

Why is it so important to choose your primer en probes precisely?

18 February 2016 3,773 4 View

Calculating amplicon size?

Hi everyone. I have a simple question, but i can't seem to solve it myself. Ive found 2 primer sequences in an article in order to amplify my region of interest. However, I can't find out how to...

18 February 2016 5,441 8 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

How long does it take to get a modeller(for homology modelling) license?

I submitted my e-mail address on modeller website to get a license. The problem is that, the site cannot process my school e-mail (ewhain.net). I submitted my Lab website and school name so that...

25 February 2021 9,054 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View