What are the advantages and limits of pollen grains in paleoclimatological studies?

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Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View

Hot to do MTT assay data analysis?

I have repeated an MTT assay three times using different batches of the same cell line. Each time, I tested the same treatment (different medium) and seeded the same number of cells. Additionally,...

08 July 2024 1,900 1 View

What is wrong with Site-Directed Mutagenesis?

I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...

24 June 2024 6,079 4 View

Does anyone have suggestions for this esterification?

I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...

24 June 2024 9,319 0 View

How do you add multiple nodes or cutting planes in Abaqus?

Hi, In a previous question I asked how to effectively model springs in Abaqus. However, I figured out the feature under Assembly -> springs/dashspots -> node to node Springs. I have a cylinder...

20 June 2024 892 3 View

How do you add radial spring elements in Abaqus?

Hi, I am trying to complete a buckling analysis on a tube with an external pressure. This solves itself easily by adding radial constraint on the internal edge of the tube, one axial constraint...

17 June 2024 8,165 7 View

Given that Mesozoic placentals were largely arboreal (with fingers), do you know of any studies that illustrate the finger-to-hoof transition?

I'm interested in the earliest days of placental terrestrial locomotion in large herbivorous taxa. Phenacodus seems to be transitional in this regard. Any others?

06 June 2024 5,182 2 View

How reliable is ninhydrin test in solid-phase peptide synthesis?

Hey everyone! I'm doing peptide synthesis on Rink amide resin and sometimes I need to add amino acids with secondary amines or beta amino acids. Normally I synthesize 35 to 40 mer peptides. As a...

21 May 2024 9,035 0 View

How does one generate a normalized TCSPC curve from actual photon counts versus time?

Sometimes TCSPC curves are analyzed using the actual photon counts, and sometimes they are normalized with the signal running from 0 to 1, or even from 0 to some value less than 1. What quantity...

20 May 2024 4,277 0 View

Problem when running Spin-Orbit Coupling using CASTEP. What is nlpot_prepare_precon_ks?

I am currently using CASTEP version 22.11, and I encounter the following error when running calculations with spin-orbit coupling: Error with dsytrf in nlpot_prepare_precon_ks Current trace...

19 May 2024 4,236 5 View

How does the idea of a proxy measure work?

I came across this idea that I've never heard of in the study of Li et al. (2023), just a brief context, it's a review on the relationship between epistemic trust and developmental...

11 June 2024 8,101 1 View

What are the most appropriate variables for measuring profitability of Robusta Coffee production by smallholder farmers using panel data?

Dear researchers and academicians. I am kindly requesting for your contributions to the above question on proxies for profitability of smallholder coffee production. Thanks so much and kind regards.

04 June 2024 722 0 View

Problem with my Proxy model.?

Hello everyone and thank you for reading my question. I have a data set that have around 2000 data point. It have 5 inputs (4 wells rate and the 5th is the time) and 2 ouputs ( oil cumulative and...

04 March 2024 2,248 2 View

Dear Profs, apart from GDP what other proxy can be use to measure economic growth with authority and define source of data?

Dear Profs, apart from GDP what other proxy can be used to measure economic growth with authority and define the data source?

23 January 2024 1,193 4 View

What's the meaning of the correlation between DCO3 and temperature?

I am learning to calibrate the paleotemperature proxy and have been reading a few papers that compare the relationship between DCO3 and temperature. I feel confused. Why should we compare these...

03 December 2023 4,980 1 View

How do I approach this in my research design?

Hello, Question regarding my Master Thesis research design and approach. I'm doing research on a dataset If earnings management has a negative relationship with the use (AND degree) of...

01 November 2023 212 2 View

Please what can i use to proxy government policy ?

For conducting research on government policy and agriculture

23 October 2023 1,376 2 View

What are the possible proxies for public sector budgeting?

17 September 2023 10,021 4 View

Which tissue is closest to the prostate gland in terms of MRI features (e.g., relaxation times) and physiological characteristics ?

As the pig prostate (our animal model) is relatively difficult to access for an ablation procedure, we'd like to use a proxy organ.

03 September 2023 4,822 0 View

How do I convert a database of 100000 molecules to 3d sructure?

Hello everyone, I am trying to convert the LOTUS database into pdbqt molecules from sdf as well as smiles. I have tried openbabel as it is the goto software. but it gets stuck if it encounters...

17 August 2023 213 3 View