What are the advantages and limits of pollen grains in paleoclimatological studies?

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When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

SPSS - Merging files by ID numbers that contain letters?

Hi, I'm trying to merge files from a data base of 20 files, each containing about 4000 subjects. Unfortunately the ID numbers are actually a combination of numbers and letters (e.g., b1458219)....

15 February 2021 5,833 3 View

How would I go about getting an expert review on the interview questions for the dissertation?

Good Evening, My name is David Marshall and I am Doctor of Management Student at Colorado Technical University Online. I just recently received approval from the IRB to start data collection on...

08 February 2021 346 7 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

How does DMSO percentage affect ELISA?

Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...

31 January 2021 6,771 1 View

Is there any publications that investigated the paleo Pacific Decadal Oscillation over million years ago?

I know some papers reconstructed the paleo PDO by proxy data. But the time periods of PDO are usually thousands of years ago. I want to know something about the PDO in deep-time geology period....

02 September 2020 7,712 3 View

What might be the proxy of "artificial intelligence"?

I am working on research related to financial economics and AI, I need the proxy of AI. Thank you

30 August 2020 9,457 5 View

Can '0' value be assigned to missing values, particularly where I am considering creating an index using PCA through some proxies?

Greetings from my side. I am working on a panel of Asian countries. However, the biggest hurdle I am facing is missing values/data for financial inclusion. In many papers of financial inclusion,...

25 July 2020 8,164 3 View

What is the duration of viral shedding in persons with coronavirus disease CoVID-19?

In a small group of patients with mild COVID-19, nasopharyngeal/oropharyngeal swabs collected during the first week of illness showed infectious virus but not after this period despite high...

23 June 2020 9,281 20 View

What programming language can we recommend to a paleoclimatology student?

I will use climatological models in my research work but I have not yet mastered any programming language, now I would like to have advice on the choices to start.

21 June 2020 9,595 9 View

What are some of the free sources where i can download paleo-climate data ? What things you take into considerations while processing paleo data ?

Greetings, I am planning to work with open source paleo-climate data for a thesis, so far the only source for these kind of data i know is : https://www.ncdc.noaa.gov/paleo-search/ Is there...

28 May 2020 9,084 14 View

Why the Adult IQ difference challenge in innovation?

Recent studies of children's tool innovation have revealed that there is variation in children's success in middle-childhood. In two individual differences studies, we sought to identify personal...

11 December 2019 4,481 3 View

How Bank Credit influence Corporate working capital management in Pakistan?

How the bank credit influence the corporate working capital management policies of commercial and industrial sector of Pakistan? What kind of different proxies (Operational Variable) would be...

29 November 2019 5,019 4 View

How Bank Credit influence Corporate working capital management in Pakistan?

How the bank credit influence the corporate working capital management policies of commercial and industrial sector of Pakistan? What kind of different proxies (Operational Variable) would be...

10 November 2019 7,911 4 View

Can you recommend methods in computing for Ecological Water Requirement (EWR) with limited or proxy data?

I am conceptualizing my dissertation in line with policies on water resource management and sustainability. I do not have a strong background in hydrology but I believe EWR is very much crucial to...

09 October 2019 7,222 3 View