please suggest culture media to improve callus growth of lentil. low callus from seeds & in vitro stems for cytokinin and auxin alone/combination in MS. Adding casein hydrolysate was little better. I also need suggestion for shoot organogenesis from callus. I am developing salt tolerant callus lines.
Try to check this article in tomato. I may contain some useful information that can answer you question
Conference Paper Molecular Markers associated with callus induction in some t...
Improvement of callus use the higher amount of IAA and lower amount of GA use. You try with the ratio 10:1. you also try with some time add the lentil extract in medium. It also work for callus induction.
Thanks a lot for the advice @ Dr. Ahmed and Dr. Kalpesh. Any suggestions regarding shoot regeneration from callus would be appreciable.
Improvement of callus use the 0.5mgl-1NAA + 1mgl-1 2,4-D or 0.5mgl-1NAA + 1mgl-1Kinetine add to MS medium.
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Plant Biology
- Plant Biotechnology