I'm looking to image some plant roots after allowing fluorescent biofilms (producing GFP and mCherry proteins) to form on the roots. I am just curious if anyone who has done this would share how they prepared the plant roots before imaging them. I had planned to put cuttings on a slide or in a glass-bottomed dish but worry about unforeseen problems.
I work with confocal but have never tried imaging plant stuff. I guess for me using a mattek dish to put my samples on is of help. I would suggest you could simply take the sample in a mattek dish immersed in a specific liquid medium (or water) and try to image them and that should work.
Hi Tj,
lightsheet microscopy is a viable option for 3d fluorescence imaging of some roots without the need to slice them. Of course it all depends on the size and optical properties of your sample. You can find a nice publication here:
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0075646
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