How can I prevent sections of adult drosophila brains from washing away from the slides during staining procedure?

I am working on 30um sections of adult drosophila brains. I dual stain the sections first with Alexa-fluor 594 anti-HRP stain and then with DAPI to stain the neurons and the nuclei...

11 December 2015 3,563 5 View

Will Eosin affect other fluorescent staining of drosophila neural tissue?

I am working on sections (25um) of adult drosophila brains - specifically their optic lobes. The sections are quite transparent and very difficult to view - so I was thinking of staining them with...

08 September 2015 3,136 2 View

Can I stain a single specimen with both Golgi staining and DAPI? Are they compatible?

I am using Golgi's staining protocol to stain my neurons (adult drosophila brains - sectioned). Since I am studying the very minute retinal neurons, I am having difficulty in picking out their...

07 August 2015 4,620 4 View

How do I dissolve the tissue freezing medium after fixing the sectioned specimens onto my slide?

I have to do post-section staining of my slides. With the tissue freezing medium present, my specimens were washed away during staining. So, how do I dissolve this medium without affecting my...

07 August 2015 4,604 4 View

In what solution can an insect, along with its nervous system intact, be preserved for cryotome sectioning?

09 October 2014 9,076 2 View

What exactly are expansion segments of the Eukaryotic ribosomes?

I need to compare two organisms to figure out if they're related but one of the organism is not well studied and only a part of its expansion segment is sequenced. Is it useful to compare the...

09 October 2014 368 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View