How can I prevent sections of adult drosophila brains from washing away from the slides during staining procedure?

I am working on 30um sections of adult drosophila brains. I dual stain the sections first with Alexa-fluor 594 anti-HRP stain and then with DAPI to stain the neurons and the nuclei...

11 December 2015 3,563 5 View

Will Eosin affect other fluorescent staining of drosophila neural tissue?

I am working on sections (25um) of adult drosophila brains - specifically their optic lobes. The sections are quite transparent and very difficult to view - so I was thinking of staining them with...

08 September 2015 3,136 2 View

Can I stain a single specimen with both Golgi staining and DAPI? Are they compatible?

I am using Golgi's staining protocol to stain my neurons (adult drosophila brains - sectioned). Since I am studying the very minute retinal neurons, I am having difficulty in picking out their...

07 August 2015 4,620 4 View

How do I dissolve the tissue freezing medium after fixing the sectioned specimens onto my slide?

I have to do post-section staining of my slides. With the tissue freezing medium present, my specimens were washed away during staining. So, how do I dissolve this medium without affecting my...

07 August 2015 4,604 4 View

What are some non-lipophilic fluorescent dyes that can be used for staining dipteran nervous system?

11 December 2014 1,937 0 View

What exactly are expansion segments of the Eukaryotic ribosomes?

I need to compare two organisms to figure out if they're related but one of the organism is not well studied and only a part of its expansion segment is sequenced. Is it useful to compare the...

09 October 2014 368 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View