Rathnavel Pandian Thangamani

7 Questions 7 Answers 0 Followers

Questions related from Rathnavel Pandian Thangamani

How can I prevent sections of adult drosophila brains from washing away from the slides during staining procedure?

I am working on 30um sections of adult drosophila brains. I dual stain the sections first with Alexa-fluor 594 anti-HRP stain and then with DAPI to stain the neurons and the nuclei...

12 December 2015 3,591 5 View

Will Eosin affect other fluorescent staining of drosophila neural tissue?

I am working on sections (25um) of adult drosophila brains - specifically their optic lobes. The sections are quite transparent and very difficult to view - so I was thinking of staining them with...

09 September 2015 3,169 2 View

Can I stain a single specimen with both Golgi staining and DAPI? Are they compatible?

I am using Golgi's staining protocol to stain my neurons (adult drosophila brains - sectioned). Since I am studying the very minute retinal neurons, I am having difficulty in picking out their...

08 August 2015 4,652 4 View

How do I dissolve the tissue freezing medium after fixing the sectioned specimens onto my slide?

I have to do post-section staining of my slides. With the tissue freezing medium present, my specimens were washed away during staining. So, how do I dissolve this medium without affecting my...

08 August 2015 4,640 4 View

What are some non-lipophilic fluorescent dyes that can be used for staining dipteran nervous system?

Besides DAPI which directly binds to the DNA, what other non-lipophilic dyes that I can employ to visualise the whole brains of adult drosophila and other diptera? I've already ruled out GFP as...

12 December 2014 1,990 0 View

In what solution can an insect, along with its nervous system intact, be preserved for cryotome sectioning?

I'm studying an insect's brain and I would like to know what would be the ideal preserving solution to keep the nervous system of the insect intact. Will ethanol or formaldehyde solution be ideal?

10 October 2014 9,130 2 View

What exactly are expansion segments of the Eukaryotic ribosomes?

I need to compare two organisms to figure out if they're related but one of the organism is not well studied and only a part of its expansion segment is sequenced. Is it useful to compare the...

10 October 2014 398 3 View