Dear Shubha,

 There are many tools to design shRNA

1. https://rnaidesigner.thermofisher.com/rnaiexpress/

2. http://dharmacon.gelifesciences.com/design-center/

3.https://eu.idtdna.com/site/order/designtool/index/DSIRNA_CUSTOM

4.http://www.genelink.com/sirna/shRNAi.asp

For more and good information about your question click this Research gate link ,

https://www.researchgate.net/post/which_is_good_online_available_software_to_design_shRNA

Best of Luck.

Dear

I can add you the following links:

Thank you

http://cancan.cshl.edu/cgi-bin/Codex/Codex.cgi

http://www.invivogen.com/sirna-wizard

http://biosettia.com/support/shrna-designer

http://www.invitrogen.com/site/us/en/home/References/Ambion-Tech-Support/rnai-sirna/general-articles/-sirna-design-guidelines.html

Great. Thank you!

Please give a look at http://shrnadesigner.org/