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Questions related from Shubha Suresh
Details: I am looking at a large structure within human cells over time and I want to obtain a cross-sectional view of the center of the structure. So, I need to first align the data sets so that...
22 August 2017 6,597 2 View
I am trying to clone and express shRNA sequences targeting a specific gene using the pSUPERIOR.Retro.Puro vector. I already have the targeting sequence and I am looking for a software to design...
28 March 2017 4,173 4 View
I want to synthesize dsDNA coding for shRNAs from two complimentary oligos, anneal them and clone it into a retroviral vector. Due to hairpin formation within the two oligos, I predict the...
28 March 2017 3,906 1 View
I tried the MUSCLE software but that cannot handle my sequences and says it is long. My protein is around 5000 amino acids long.
16 March 2017 3,486 4 View