Shubha Suresh

4 Questions 5 Answers 0 Followers

Questions related from Shubha Suresh

Expertise in alignment and analysis of a time-lapse data set in 3D?

Details: I am looking at a large structure within human cells over time and I want to obtain a cross-sectional view of the center of the structure. So, I need to first align the data sets so that...

22 August 2017 6,602 2 View

What are some good softwares for shRNA design?

I am trying to clone and express shRNA sequences targeting a specific gene using the pSUPERIOR.Retro.Puro vector. I already have the targeting sequence and I am looking for a software to design...

28 March 2017 4,177 4 View

Are there recommendations for shRNA cloning protocols?

I want to synthesize dsDNA coding for shRNAs from two complimentary oligos, anneal them and clone it into a retroviral vector. Due to hairpin formation within the two oligos, I predict the...

28 March 2017 3,910 1 View

I want to construct a phylogenetic tree based on my protein of interest (that is huge). Recommendations for softwares that can handle long seqs?

I tried the MUSCLE software but that cannot handle my sequences and says it is long. My protein is around 5000 amino acids long.

16 March 2017 3,490 4 View