Do you think that all the journals, which claims ownership of impact factor is considered scientifically solid ?

Do you think that all the journals, which claims ownership of impact factor is considered scientifically solid and deserves for publication of scientific papers?.

10 November 2014 5,144 1 View

What are the recent Immunological Diagnostic techniques for Human Leptospirosis ?

10 November 2014 4,383 4 View

What are the recent Immunological Diagnostic markers for rheumatoid arthritis ?

What are the recent Immunological Diagnostic markers for rheumatoid arthritis ?

10 November 2014 9,490 2 View

What is the real correlation between migration inhibitory factor (MIF) and Cancer ?

what is the real correlation between migration inhibitory factor (MIF) and Cancer ?

10 November 2014 9,814 1 View

What are the recent Immunological markers for multidrug resistant TB cases?

What are the recent Immunological markers for multidrug resistant TB cases?

10 November 2014 3,989 4 View

Why antibody-mediated immunity to Shigella dysentery requires several episodes of infection to get primed?

Why antibody-mediated immunity to Shigella dysentery requires several episodes of infection to get primed?

06 November 2014 3,033 1 View

What are the recent Immunological markers for multidrug resistant TB cases?

What are the recent Immunological markers for multidrug resistant TB cases?

02 November 2014 6,226 3 View

How can we determine a lethal dose of plant extracts applied on cell culture ?

09 October 2014 7,872 3 View

What are the best methods for minimizing DNA contamination during extraction procedures?

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09 October 2014 6,136 4 View

What are the best methods for the differentiation of inflammatory cells from immature sperm in clinical laboratory investigations?

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09 October 2014 3,542 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View