Should GFP sequence be cut together with the IRES sequence?

Hello. I have a viral plasmid which contains a bicistronic region: CMVprmtr-GFP-IRES-PuroR. I would like to cut out the GFP sequence to get rid of the green fluorescence. In this case, would it be...

02 February 2021 1,644 7 View

Examples of SWAT used in water management in operational practice?

Do you know any examples when the hydrological model made using SWAT became some software product used in operational practice for water management (cases of the water reservoirs management are...

04 June 2020 4,191 1 View

What are these cells with highly condensed basophilic nuclei and a light cytoplasm between proximal tubule epithelial cells in the zebrafish kidneys?

Please help determine what cells are in the proximal tubules of zebrafish. They have highly condensed nuclei, but I suspect that this is not karyopiches of apoptotic cells, but mitosis of normal...

03 June 2020 4,093 5 View

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home?

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home (freeware)? Chromatograms were made by Waters UPLC I-Class PDA-MS. At the workplace,...

14 May 2020 5,383 3 View

No title

It is quite difficult to find these enzyme preparations at that moment. Can anyone tell me who I can contact on this issue with?

29 February 2020 2,130 3 View

How to dilute fish blood plasma?

We are encountered with a phenomenon when T3 FREE was estimated in fish plasma by ELISA (DRG tests): some samples are shown no significant differences in total and diluted plasma (in PBS pH7.4,...

29 September 2019 8,819 3 View

Does anybody know a database on fluorescent properties of chemical compounds?

Does anybody know a database on fluorescent properties of chemical compounds? There are many papers describing flurescent properties of various compounds. Is there any database on fluorescent...

06 August 2019 9,239 3 View

How shoud one switch off a rotary vane pump?

How shoud I switch off a rotary vane pump? Should I initially turn off the pump and then open the shutter to normalize the pressure? Or the order of the actions should be vice versa?

06 August 2019 9,982 3 View

Can anyone verify whether this is an artifact or a point mutation in these Sanger sequencing results?

Hello! I have really strange results in my sequencing chromatograms. The goal was to analyze a point mutation which is heterozygous (DNMT3A gene, in position R882). So I performed a Sanger...

17 May 2019 7,386 3 View

Good day! Can someone help me? How can the mixing speed be taken into account in the mathematical model of diffusion in a liquid medium?

Good day! Can someone help me? How can the mixing speed be taken into account in the mathematical model of diffusion in a liquid medium?

19 March 2019 7,162 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View