How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

Hello, can someone help me doing my research please...?

02 January 2021 4,065 15 View

How to define least effective concentration (LEC) and maximum safe concentration (MSC) of drugs?

In drug delivery, for efficacy, the drug concentration needs to be within LEC and MSC. How can we define that concentration?

25 November 2020 6,478 3 View

Who can help me Level of Anxiety during Pandemic Questionnaire?

Hello. I am a third-year student at the University of Cordilleras. I was hoping if you could help me regarding a questionnaire in regard to the level of anxiety during pandemic. I just need...

30 September 2020 756 3 View

Perception on business failure prediction models using the annual report?

27 July 2020 1,141 2 View

Any suggestions for tools to analysis, plotting and tabulating genomic data of bacteria genomes?

Hello, In order to analyse NGS results, I looking for some software or tool to tabulate the entire genome of a bacteria. Thanks in advance

27 July 2020 9,826 5 View

Dissertation Research: Business failure prediction. Could you help me with a short survey please?

I'm conducting a research study titled 'Perception on the business failure prediction using the annual report' to receive the degree of M.Sc. Accounting and Finance by Griffith College in Dublin,...

21 July 2020 8,761 3 View

Why does a small peptide precipitates in contact with albumin?

06 January 2020 8,673 5 View

Any analytical method for dating taxidermies or skeletons in a zoological collection?

One recurrent topic when managing zoological collections is that many specimens has not information of date when it was collected, dead or prepared. This information (besides the collecting place...

17 July 2019 5,482 3 View

Does somebody have experience with the cell line H1666 (lung adenocarcinoma)? Do they grow in RPMI-1640 media?

I am trying to thaw the cell line H1666 from ATCC. It is recommended to use DMEM F12 supplemented with lot of things (EGF, insulin, etc). Is the RPMI 1640 media also suitable? Thank you in advance

27 November 2018 5,701 2 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Reaction condition to convert NH2 to COOH?

I need to couple Dopamine by NH2 end to silica nanoparticles coated by PEG-NH2, but first i need to convert the PEG-NH2 to COOH by using succinic anhydrous ... can you suggest a reaction condition ?

13 February 2021 2,768 4 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

Coupled hydric and mechanical equation COMSOL?

hello i am trying to study the mechanical deformation due to the humidity on a 3d volume formed of 2 phases. my equation are dw/dt =grad(Dm*div(w)) and the mecanical is sigma=E*beta*nabla(w)....

27 January 2021 5,201 1 View

In abaqus, how to couple a reference point to a discrete rigid body?

I have two discrete rigid bodies. A axial connector was established to connect two reference points. Now the task is to attach the reference points to the respective rigid part. How should I do...

25 January 2021 5,079 3 View

Is it possible to determine % K2O of organic manures by STPB method ?

To determine % K2O of organic manures, flame photometric technique is used whereas in inrganic fertiliser for K2O determination STPB method is used. I am trying to find out any method of...

24 January 2021 4,714 3 View

How exactly modes are coupled into asymmetric directional coupler?

Currently I am reading one research paper regarding silicon based two and four mode division multiplexing for optical data transmission in 2 micrometer waveband. While reading I stuck at one...

21 January 2021 5,864 4 View

How to simulate Sloshing water using Dynamic mesh in ANSYS Fluent ?

I am trying to model sloshing water in a tank in ANSYS Fluent using Wall motion. BUT it seems that System coupling is not responding to wall motion and it works only with Dynamic mesh.

20 January 2021 2,963 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View