i want design a powerful signal peptide which can secrete proteins out so i need to know which signal peptides are exist to this porpose . then study them and design my signal peptide.
The MOST powerful signal peptide has probably not been found yet, but you can look at ice nucleation protein from plant pathogenic bacteria. It is one of the best.
Li, Qianqian, et al. "Molecular characterization of an ice nucleation protein variant (inaQ) from Pseudomonas syringae and the analysis of its transmembrane transport activity in Escherichia coli." Int J Biol Sci 8.8 (2012): 1097-1108.
The protein itself will have a lot of impact on its ability to be secreted. So a very strong signal peptide may not be universal.
Which organism are you using? If you are expressing your protein in E. coli, then adding a signal sequence would result just in periplasmic expression, not protein secretion to the media. If that's your goal, the typical signal sequence used if the one from OmpA.
thank you all for your useful and beautiful answers.
i want collecting the best signal peptides, study them and then design the best signal peptide to my protein, lead the protein to periplasmic expression from sec pathway in E.coli. so i need find powerful signal peptides like OmpA from any micro-organisms to study on their sequences. if you know some signal peptides like them please Introduce me.
thank you
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Microorganisms
- Bacteria