I intend to conduct this study in an undergraduate setting. And I will be using Mus musculus for this. What are cost-efficient ways to do such an analysis?
PCR can be used to measure the expression at the transcriptional level. Western blot can be used to assay the concentration of your target of interest at the protein level. PCR is usually the less expensive of the two. What do you want to do?
Thank you for your answer! I want to study the concentration levels of a particular protein within the mouse's cerebrum. I was wondering if there are any possible alternatives to Western blot, for the very reason that it is expensive to conduct.
Western blot is about as cheap as you can get for protein analysis, however, I am always dubious of using it for so called "quantitative Western", typically based on densitometry or fluorescent tags. I would personally opt for an ELISA (enzyme-linked immunosorbant assay). It generally requires less technique optimization than western, and is comparable in price (assuming you have a spectrophotometer available that can read it). The company PeproTech has a variety of ELISAs available for quite low costs.
Cheers,
Zack
- Badges
- Science method
- Similar topics
- Biological Science
- Genetics
- Gene Expression
- Protein Expression