We have staphylococcus aureus bacteria treated with ciprofloxacin attached to a gold nano-particle with a radio-labeled chelator. we want to measure the antibiotic conc uptake by bacteria, what methods can be used?
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There are many experimental methods in measuring the quantity of antibiotic uptake in a bacterium; namely, with the use of bioassays, fluorometric detection, radiometry, and HPLC. With regards to the attachment of the antibiotic and microorganism to a gold-nanoparticle with a radio-labeled chelator, the method of deep ultraviolet fluorescence (DUV) may be fit to this. The use of ciprofloxacin as an antibiotic was utilised in the example for the method, wherein DUV was used to study the drug accumulation by MDR bacterial suspensions. However, observations of interferences that may affect the result of the antibiotic uptake are noted, which is referred to as autofluorescence.
Reference:
Cinquin, B., Maigre, L., Pinet, E., Chevalier, J., Stavenger, R. A., Mills, S., Réfrégiers, M., & Pagès, J. M. (2015). Microspectrometric insights on the uptake of antibiotics at the single bacterial cell level. Scientific reports, 5, 17968. https://doi.org/10.1038/srep17968
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