I plan to use PEGDA in a microextrusion-based 3D bioprinting system. What are the methods that I could use to initiate 'reverse-gelation' of UV-crosslinked PEGDA?
Bhuvaneshwari Karunakaran I wasn't able to find a suitable method. In any case, we started using other hydrogels for our application here for unrelated reasons, and I never did try to find out possible degradation methods for PEGDA. Although, I have a hunch that using a Collagenease solution might work, even if it probably would be less efficient for PEGDA compared to other hydrogels like Gelatin etc.