How to do interdisciplinary research combining literature and visual arts?

I am a first-year PhD student who would like to hear about other researchers' experience related to interdisciplinarity within the humanities, especially those combining both text and visual...

26 May 2020 2,889 37 View

What is the best method to store sectioned FFPE slides for long term storage?

I will be having FFPE blocks cut onto slides, I would like to know what would be the best method to store slides for long term storage. I don't want the slides to go bad. I am worried about...

16 January 2019 9,208 2 View

No title

28 September 2018 7,258 4 View

What are good control tissues for a MDSC Immunohistochemistry panel?

Hi Everyone, I am currently working on a human MDSC immunohistochemistry panel using CD33, CD11b and HLA-DR. I need to find good control tissue. I tried to use normal brain and spleen tissue...

23 September 2018 4,882 3 View

Can anyone recommend a sweetener that increases salivary secretion without disturbing original blood glucose levels?

To collect salivary samples (about 1 ml) from spider monkeys. The samples will be analyzed to determine cortisol, melatonin, serotonin and testosterone through ELISA tests. Thanks for any...

06 July 2018 3,605 3 View

Is it possible to detect serotonin levels in salivary samples through ELISA tests?

The technique is considered for a project to study physiological circadian patterns in free ranging spider monkeys, and thus the best suited sample source is saliva, because of its availability...

11 December 2017 5,978 2 View

Does anyone know how to calculate the number of peptide bonds of a protein after hydrolysis?

Need that calculation to obtain the degree of hydrolysis.

07 August 2017 6,463 0 View

Which ANTS/DPX concentration should I use to see solute leakage from liposomes?

I want to see how ANTS fluorescence in liposomes changes when adding triton.My idea is to encapsulate ANTS and its quencher DPX in the same liposome so that when adding triton fluorescence signal...

27 July 2017 9,640 1 View

How can I avoid nanoerythrosomes aggregation?

15 March 2017 10,022 6 View

Can orange/blue loading dye or green master mix can cause anomalous bands in my electrophoresis from a purified PCR product?

I am trying to purified a PCR product from gel extraction (2% agarose), for the purification I used PureLink Quick gel extraction and PCR purification combo kit (Invitrogen). And for the PCR...

28 September 2016 9,012 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View