What are general principles for double-strand RNA (dsRNA) synthesis?

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Mohammad Hassan Jafari

Hi,

I think the attached article can answer your question

be happy

Mohammad Vatanparast

Dear mohammad

Thank you

Hesam Mahmodi

Hi Dear Dr. Vatanparast

What are general principles for double-strand DNA (dsDNA) synthesis?

I have designed a ds DNA sequence to use as positive control for my qPCR assay, I put my forward and reverse primer sequences at start and the end of my synthetic DNA.

Does it make any problem during annealing? or it's better to add more nucleotides before my primers?