Hesam Mahmodi

4 Questions 6 Answers 0 Followers

Questions related from Hesam Mahmodi

Anyone has primer probe sequences for H.pylori antibiotic resistant genes?

Hi, I am looking for primer/probe sequences for detection of antibiotic resistant genes in H.pylori by Real time PCR dye based. I need following list of primer probes.(Please, attached the...

14 January 2023 6,990 0 View

I see amplification in negative samples while it's not contamination. Did you have the same problem and how you solved that?

Hi I'm faced a problem on performing Real time PCR on RNA. Amplification happened in negative samples as showed in the picture but it is not primer/dimer phenomenon.(by primer/probe dilution...

28 October 2022 8,224 3 View

I want to set an OD to read by Microplate reader on 384 or 96 well plates. anyone has the same experience on this topic and. how you set that OD?

I want to set different ODs as blank for different antibiotic dilutions on 384 well plate and measure it after 24 hours and report MIC 50% (Minimum Inhibition Concentration) automatically by OD...

17 March 2022 6,878 3 View

An issue during amplification of H.pylori resistant genes A2142C, GyrA(271T),GyrA(272G), GyrA(261G) and GyrA(271A)

Hello, I have ordered A2142C, GyrA(261A), GyrA(271T),GyrA(272G), GyrA(261G) and GyrA(271A) primer probes in this literature separately. Evaluation of multiplex ARMS-PCR for detection of...

01 January 1970 3,073 0 View