What are the possible reasons for getting bands for both methylation and unmethylation primer set in MS PCR?

I am currently doing methylation specific PCR and I find that all my samples are showing bands for both methylation and unmethylation in both the control and tumor samples. I wonder is it because...

22 September 2020 6,002 4 View

How to run Two-way PERMANOVA mixed in R? And do pairwise?

My experiment has two factors, binary and continuous variables. The normality test gave p

17 February 2019 5,620 3 View

Problem with RT-PCR. Enzyme alone with RNA, in RT, works. Why?

Hello everyone, I was just trying to do an RT-PCR with specific oligos to select the correct strand that I want to study. I cannot distinguish between both strands. So, I have tested different RT...

20 September 2018 1,680 3 View

How to transfect bone derived human mesenchymal stem cells with siRNA?

I am trying to transfect human mesenchymal stem cells with siRNAs. I've tried it with lipofectamine method but it didn't work. However, in other cell lines (like293T), it works. Is there any...

13 July 2017 1,034 2 View

How to analyse a CD spectra of proteins to get secondary elements proportion?

I have been measuring circular dichroism spectra between 250 and 190 nm for proteins. I would like to calculate the percentage of each secondary structure element . Does anyone know any software...

02 March 2017 6,647 3 View

How to measure small peptide concentration?

03 April 2016 257 17 View

How to measure small peptide concentration?

31 March 2016 9,907 17 View

Which is the best way to study interaction between protein and antibody?

I'm trying to study the recognition sites of specific antibodies. For that, I have expressed the recombinant protein and different fragment of it. I have human serum with antibodies againts this...

03 October 2013 5,886 6 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

Are cloud points able to identify tree species?

Hello Dears My goal is to classify dense forest tree species using drone images. Is it possible to classify dense cloud points? If yes, in what way and in what software? Thankful

15 February 2021 3,475 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Global Warming effects?

Hi Professionals! Due to Global warming the earth temperature increases (https://climate.nasa.gov/effects/), which generates floods, storms, and tornadoes because of melting of ice glacier, in...

08 February 2021 9,038 8 View

Are there any papers that deal with the effects of climate change on the pollination in Tropical orchids?

There are a few papers dealing with the effects of climate change on the pollination of European orchids, but I wonder if similar studies have been carried out in the Tropics.

04 February 2021 1,818 4 View

Is there any procedure or an equation for converting total carbon to organic carbon or any equation that helps to obtain the value?

I have analysed percentage carbon (total) using CHNS Elemental Analyzer for the forest soils. Now, I would like to obtain SOC content from the obtained value for which I am looking for a procedure...

29 January 2021 5,551 8 View

How to plot Random Forest model as raster prediction map in RStudio?

Output_raster

27 January 2021 7,974 3 View

What is the magnitude of yearly rhizopdeposition in tropical forests?

Hi all, I am trying to find reference values on annual soil rhizodeposition rates for different kinds of forests, with a particular interest for tropical rainforests. Do we have an idea of the...

26 January 2021 4,278 4 View