see

https://pdfs.semanticscholar.org/bb7c/9b1466e9be84feaad1e824966044820551a8.pdf

http://www.intbrazjurol.com.br/pdf/vol3139/Vol32_n6_2006.pdf

http://www.qums.ac.ir/portal/file/?180440/Principles.of.Surgery.Companion.Handbook.7th.ed.3HAXAP.pdf

Jumaa Salman Chiad Thank you for your response.

However, I was not seeking extensive literature about surgical methods, but specifically, some tips about the dissection of the seromuscular layer in the murine intestine. This is because the mouse intestine is very small and thin, and the dissection of the seromuscular is a hard task, differing from dissecting a pig or human intestine.

It would be very useful to receive specific tips and "tricks" from a person who has already stripped a seromuscular layer from a rat or mouse intestine, or who is familiar with this technique.

Cheers,

Javi

Hi Javier,

I have done many Ussing experiments on rodents intestines in my ex-lab (INSERM U913). We used to keep the entire wall for this type of experiments (paracellular and transcellular permeability) when we worked on rodents tissues. But if it's a problem for your experimental design or parameters you want to look at, the best way is to get mucosal strip like you try to do. I know that it's tricky and depending of the animal, the intestine segment you use... For my experience, I'll fix the tissue on silicon-based matrix like you do, mucosa on top and I'll use FST forceps to open mucosa on a side and on a certain lenght and after I'll use a FST curved forceps to strip the mucosa. You'll have to try different orientation to strip your mucosa until you find the good direction to strip correctly the mucosa. It still really hard to get clean and not scratched mucosal strips which is needed for Ussing. With practice and patience, you can achieve that. But it's not really adapted when you have a lot of animal tissues to process, use of the entire wall is in this case recommanded.

Good luck in your research!

Julien