What are cell surface markers of activated NK cells?

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Why do some of my Raman spectra show no signal at lower Raman shifts?

I'm using a InVia Renishaw Raman microscope system (785nm wavelength, 600l/mm grating density) to acquire Raman images on human brain samples, at 50um resolution. The samples are placed on MgF2...

30 July 2024 8,771 3 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

COMSOL simulation of transient wave optics propagation is not physical?

Hi guys, I am trying to simulate a 1 ps long plane wave propagating in a dispersive material, with the wave optics transient module. According to the drude-lorentz model, when the frequency of...

29 June 2024 486 1 View

How to removal SDS/SLS from large quantities of water?

Hi all, I have a several gallons of water mixed with low percent SLS, how can i remove the SLS in a cost-effective manner.

24 June 2024 6,725 3 View

Is grammaticalization/subjectification a mechanism or motivation?

I know that we can categorize a specific linguistic change as either gramaticalization or subjectification (or both involved), but can we also say that specific linguistic change is motivated by...

31 May 2024 2,106 2 View

Clarification on 3 x 2 factorial designs and reporting?

Hi, I am currently looking at a study where they used a 3 x 2 factorial design where they had 3 diets (A, B, C) and 2 enzyme supplementation levels (0 vs 1 %). Thus giving 6 treatments. Authors...

26 May 2024 4,626 1 View

Factorial Design Experiment Data in a Meta-Analysis?

Hi, I am currently creating a meta-analysis on the effect of growth performance of pigs in response to dietary fiber content in experimental diets as well as the influence of a fiber enzyme...

25 May 2024 8,855 1 View

The hymen in sexually active women can be intact or not?

Examination of sexual assault case and giving evidence in legal situations

14 May 2024 5,228 3 View

What are the most critical construction site hazards in Ghana that a mobile app could effectively monitor and report on to improve safety outcomes?

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09 May 2024 5,316 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

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Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

State of art in natural disasters?

Are increasing the costs of disasters in the affected countries.

01 August 2024 1,794 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Jitendra Kumar Shandilya

Dear Chris,

I hope you will find your answers in thepapers attached.