Can enzyme that is extracted from Aspergillus niger be used in the range of 4-40% v/v for optimization of veggie juices like broccoli??

09 December 2020 7,083 3 View

Genomic DNA Exteraction from Asp nidulans?

Hi All, I have several trials for few months to extract good genomic DNA from some cultures of Asp. nidulans, most of them worked with CTAB extraction from freez-dried mycelium (30-40-60 hrs old)...

21 October 2020 6,030 3 View

Calcofluor white staining works with Aspergillus niger spores?

I'm trying to obtain colonies with only mycelia and without spores. I know you can use the calcofluor white staining for the fungi because it binds the cell wall of spores and it's fluorescent....

01 August 2020 6,952 2 View

How many references sequences should use for a phylogenetic tree construction?

I have identified fungus isolates from the fruit surface by SSU +ITS+ LSU regions, such as : 1) Fusarium kyushuense , 2) Fusarium proliferatum ;3) Aspergillus aculeatus ;4) Aspergillus europaeus...

15 July 2020 5,573 2 View

Do lower nanoparticle concentrations give better results than higher concentrations?

When using one of the nano particle to inhibit the growth of the fungus Aspergillus flavus on the culture medium PDA, the lower concentration gave better inhibition rates than the higher...

07 July 2020 7,722 11 View

How old is the "fungal isolate" most suitable for pathogenicity testing on the surface of fruits?

I have different fungus isolates such as; 1) Fusarium kyushuense , 2) Fusarium proliferatum ;3) Aspergillus aculeatus ;4) Aspergillus europaeus ; 5) Aspergillus flavus ; 6) Penicillium...

01 July 2020 6,889 3 View

What can be the presumable reason for the lower activity of cellulase at 5mM concentration of metal ions than at 2 &10mM concentration ?

I have purified enzymes cellulase and beta-glucosidase and performed SDS-PAGE. Therefore, I have characterized with metal ions at 2mM, 5mM and 10mM but at 5mM concentration lower activity than at...

25 March 2020 8,056 3 View

How are digestive enzymes for human consumption commercially produced?

Hello, Many protein shake powder mixes contain digestive enzymes such as protease, lipase, and cellulase. However, the products do not detail from which organism the proteins are produced or...

21 March 2020 5,048 3 View

Is the genus Neosartorya one of the telomorphs of the Aspergillus genus? Are there other telomorphs of all species of Aspergillus?

While discovering the types of Aspergills, I found a colony in white color that has microscopic structures similar to Aspergillus microscopic structures. When I used the Aspergill keys he...

07 March 2020 1,605 3 View

How do I measure collagen deposition in asthmatic mouse lung using ImageJ?

Hello All I am working on allergic asthma and hyper-responsiveness in mouse exposed to Aspergillus fumigatus. I am trying to measure collagen deposition using the ImageJ software but i don't know...

02 March 2020 6,830 3 View