What causes low yield of Cellulase Production from Aspergillus Niger using Mandels and Weber 1969 production recipe? I'm only getting 0.13 FPU/ml using Adney and Baker 2008 protocol.
I would like to know the minimum amount of Cellulase enzyme that could be used for silage making.
03 August 2024 3,170 1 View
In the year 2022 the WHO released a fungal priority pathogen list in which Cryptococcus neoformans being placed at the top most of critical priority pathogen. Is it still a threat to human...
10 June 2024 4,055 3 View
Greetings everyone, I am currently tasked with conducting DNA extraction from Aspergillus isolates, utilizing the QIAgen genomic buffer set kit. Regrettably, not all the components outlined in the...
03 May 2024 4,985 0 View
We are performing CRISPR on a gene from a strain of Aspergillus niger and then sequencing the results by Sanger. When we compare the gene sequence with the resulting sequence we observe in the...
25 April 2024 6,159 0 View
Hello, everyone. When I use CICC 40553 Aspergillus niger (strain introduction can produce citric acid) to ferment corn meal and glucose, the fermentation condition is 30 ℃, 200 rpm, and the...
29 March 2024 9,299 4 View
Good day, I hope everyone is well. I am looking for the correct method to calculate the required volume of enzyme to add to my flasks in order to perform enzyme hydrolysis in the conditions I am...
22 March 2024 3,382 0 View
Cellulose is broken down by the enzyme cellulase, to find the cellulase activity and unit production by the microorganisms, the microorganisms were cultivated by using cellulose substrate CMC and...
13 March 2024 1,390 0 View
I have to inoculate 6 discs of Trichoderma and aspergillus species in Potato dextrose broth in order to make the cell pellets of them .How do I measure or know the size of fungi discs .
13 March 2024 991 3 View
I'm working in a project where I need to test the enzyme activities of an insect's gut fluid. I've already tested it and found some good activity. Now I want to know whether the enzyme, showing...
23 February 2024 9,561 1 View
This could involve manipulating media composition, fermentation conditions (pH, temperature, oxygen), or even strain engineering approaches.
12 February 2024 2,232 1 View