Christian Ibarra

6 Questions 6 Answers 0 Followers

Questions related from Christian Ibarra

Is there a good protocol for measuring ascorbate levels in plant leaves?

Hi All, I need to measure the ascorbic acid redox state [Asc]/[DHA] in plant leaves. I’m wondering if anyone has any decent protocols for this? Best regards, Christian

27 January 2022 9,918 3 View

What are all the known nitrogen transporters in corn (maize)?

Hi All, I have some compounds and I'd like to examine how they effect the expression of nitrogen transporters in maize (corn) roots. My plan is to grow corn in a defined media (i.e. Yoshida's) in...

29 June 2017 4,811 2 View

What is the best total plant (corn leaves) RNA extraction kit to use for qPCR?

Hello All, I'm wondering what's the best plant total RNA extraction kit to use, if the down stream assay will be qPCR? Also this is for corn leaves and roots. I've been using the Spectrum (TM)...

25 April 2017 829 2 View

How can you use imageJ to determine the amount of red dye in a leaf?

Hi Folks, I'm doing a very simple assay which involves placing plant cuttings in a red dye solution. The dye can then be seen in the leaf (due to transpiration). I'd like to try and make this...

08 February 2017 1,276 4 View

Can someone please suggest a simple assay/experiment that i can use to test how plants use up water?

Hi All, I really need an assay(s) that can test how efficient a plant is using water? Thus far, my method for measuring this have been by using a porometer, and a simple assay where you put plant...

11 January 2017 5,102 6 View

Is there a phloem-specific stain (dye) for barley?

I'm guessing a phloem-specific stain for grasses would work as well. I have a few barley lines carrying a phloem-specific reporter (DsRED), but I want to make sure the expression is indeed coming...

20 February 2014 9,829 4 View