Staphylococcus species isolated from canary bird.
Performed PCR for 16rRNA for initial screening.
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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
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Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
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I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
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