I am working on the C. elegans neurodegenerative model. I am doing drug analysis against them. When I do do its western blot by using sigma monoclonal antibody at different conc. I don't get my desired bands at 4kDA. If I increase the conc of primary antibody, it gives a alot of non specific bands.
If have also used 4G8 antibody but no luck.
Can any body help me in this? Your suggestions are highly welcomed