How to incorporate a variable in damage model?

15 December 2020 7,284 2 View

Mathematical or computational modelling of protein oxidation?

I am looking for a research paper about the mathematical or computational modelling of protein oxidation (caused by reactive oxygen species).. I would really appreciate that if someone helps me...

17 November 2020 2,340 3 View

Can FEM model learn from experimental results?

I am looking for a research in which the finite element model was trained based on experimental results.

02 October 2020 9,277 19 View

Master thesis about the field of the luxury watch market?

18 September 2020 5,802 5 View

For connection between the bolt to the steel element, which kind of interaction should be considered on ABAQUS?

This design is to check for rupture in the steel element and the behavior of the bolt does not matter.

14 August 2020 6,580 2 View

Is it possible for anybody to help me for fix bridge modeling in OPENSEES?

I modeled a two-span concrete box girder bridge base on FHWA design specification, and now I recognize a problem in modeling because my first period is higher than I expected. With initial...

12 July 2020 415 3 View

Why does stresses evolve in only one element in Abaqus explicit?

I am trying to run my VUMAT code with a model in Abaqus/explicit. After that the run has been completed, I observed that the stresses have been evolved in only one element as shown in the attached...

27 May 2020 5,506 3 View

How to do baseline correction for DSC test data?

I have some raw DSC test data and I need to do baseline correction. I don't know how should I do it. I would appreciate it if someone can help me with this.

16 May 2020 8,273 7 View

Problem with nonlinear curve fitting?

Hello all, Actually, I am dealing with an issue that I can't get desirable results out of my curve-fitting problem. I used lsqcurvefit in the first place. Then, I added MultiStart to my code as...

30 April 2020 4,943 2 View

Tips on how to implement organic computing?

I'm researching the Self - Adaptive System of hospital systems.But I've had trouble figuring out how to do organic computing for self-adaptation in hospital systems.I am looking for an article or...

14 April 2020 1,743 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View