11 November 2018 8 5K Report

Hello,

So, first I perform immunoprecipitation. The eluate is then subjected to immunoblot analysis using an antibody against a related protein (equal size) and gives a band.

How sure am I the eluate is pure ? I've tested the IP eluate on western blot with antibodies against random proteins (for example Actin), and this does not give a signal.

Thanks a lot

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