Stijn Vdb

11 Questions 15 Answers 0 Followers

Questions related from Stijn Vdb

Immunoprecipitation: beads without antibody binding still give a signal?

Hi all, As a negative control, I've used a set-up in which: 1) beads without antibody binding were incubated with the sample and then put on WB. I don't see any heavy chain (which is logic),...

02 February 2020 7,366 2 View

Slower migration on SDS-page in complex sample?

Hi all, Is it possible that the migration of my protein is delayed due to the complexity of the sample? When I run a western blot with 1) a protein extract from locust tissue and 2) hemolymph...

06 June 2019 2,842 11 View

Secondary antibody gives same signal as primary?

Hi, After immunoprecipitation and elution, I check the eluate with western blot. If I incubate the blot ONLY with secondary antibody, it gives the same signal as incubatin with primary &...

01 January 2019 1,180 5 View

Western blot on immunoprecipitation eluate detects related protein?

Hello, So, first I perform immunoprecipitation. The eluate is then subjected to immunoblot analysis using an antibody against a related protein (equal size) and gives a band. How sure am I the...

11 November 2018 4,612 8 View

Allways same (wrong) sequence after sanger sequencing?

Hi, I've cloned and sequenced my PCR product but, besides a good run, I allways find the same (wrong) sequence in other runs. Running a BLAST gave no result. What could this be?? Thanks!

02 February 2018 3,549 0 View

Agilent Bioanalyzer miRNAs of 35nt?

Hi, My sample contains miRNAs, but when I use the small RNA chip (Bioanalyzer Agilent), I get a peak at around 35 nt instead of 22 nt? I'm pretty sure that there are miRNAs in there; any...

11 November 2017 7,692 4 View

Only cds on NCBI?

Hi, When you look up an mRNA seq of a certain gene, it says "complete cds" on NCBI. This means that only the coding sequence is given, right? When you compare this mRNA seq to a NCBI TSA...

09 September 2017 7,611 2 View

How to separate a complex fluid in a vesicular and non vesicular part

Hello, Is it possible to separate a complex fluid in a vesicular part (containing vesicles / exosomes) and a non - vesicular part, containing other proteins? Many thanks!

06 June 2017 2,901 4 View

What is the function of XBP buffer in exosome isolation?

Hello, What is the function of XBP buffer in exosome isolation? Does it solely bind to exosomes, or also other protein complexes?

05 May 2017 1,184 2 View

Synthesis of RNA mimics/antagomirs?

Hello, I was wondering how RNA mimics and antagomirs are being synthesized. I don't need the details (volumes etc), but just a general overview of enzymes being used etc? Thanks! Cheers

10 October 2016 2,513 1 View

Different elution methods for immunoprecipitation result in different bands on western blot?

Hi all, When I do the exact same IP protocol, but elute with different buffers (for example, in 50 µl LDS or LDS together with sample reducing agent and MQ water, or in 1X Laemli), I get...

01 January 1970 6,031 6 View