Hi,
I've cloned and sequenced my PCR product but, besides a good run, I allways find the same (wrong) sequence in other runs. Running a BLAST gave no result. What could this be??
Thanks!
Hi all, As a negative control, I've used a set-up in which: 1) beads without antibody binding were incubated with the sample and then put on WB. I don't see any heavy chain (which is logic),...
01 February 2020 7,426 2 View
Hi all, Is it possible that the migration of my protein is delayed due to the complexity of the sample? When I run a western blot with 1) a protein extract from locust tissue and 2) hemolymph...
05 June 2019 2,905 11 View
Hi, After immunoprecipitation and elution, I check the eluate with western blot. If I incubate the blot ONLY with secondary antibody, it gives the same signal as incubatin with primary &...
31 December 2018 1,218 5 View
Hello, So, first I perform immunoprecipitation. The eluate is then subjected to immunoblot analysis using an antibody against a related protein (equal size) and gives a band. How sure am I the...
10 November 2018 4,739 8 View
Hi, My sample contains miRNAs, but when I use the small RNA chip (Bioanalyzer Agilent), I get a peak at around 35 nt instead of 22 nt? I'm pretty sure that there are miRNAs in there; any...
10 November 2017 7,746 4 View
Hi, When you look up an mRNA seq of a certain gene, it says "complete cds" on NCBI. This means that only the coding sequence is given, right? When you compare this mRNA seq to a NCBI TSA...
08 September 2017 7,658 2 View
Hello, Is it possible to separate a complex fluid in a vesicular part (containing vesicles / exosomes) and a non - vesicular part, containing other proteins? Many thanks!
05 June 2017 2,950 4 View
Hello, What is the function of XBP buffer in exosome isolation? Does it solely bind to exosomes, or also other protein complexes?
04 May 2017 1,239 2 View
Hello, I was wondering how RNA mimics and antagomirs are being synthesized. I don't need the details (volumes etc), but just a general overview of enzymes being used etc? Thanks! Cheers
09 October 2016 2,579 1 View
Hi all, When I do the exact same IP protocol, but elute with different buffers (for example, in 50 µl LDS or LDS together with sample reducing agent and MQ water, or in 1X Laemli), I get...
01 January 1970 6,076 6 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View