Somebody have experience with the WES proteinsimple system? and it is better than traditional Western blotting? and how expensive is (reagents, antibodies, etc.) it?
Thanks a lot in advance
We had the early version of the Protein Simple system. This was the one in which all capillaries were to be prepared manually. But I think the separation steps work the same.
Anyways, the system is good for doing 'assembly line' type work where one is using a small set of pre-characterized antibodies and cells (lysates). if you are going to be repeating the same type of detection day after day, then this system is perfect. Although, you still have to find the best conditions for the Ab and sample, if these two are the same all the time, then you can run many assays for e.g an experiment where you just change the treatment conditions of cells and look at the same protein.
We did not find it convenient enough to be able to replace Western blotting. (a). The cost of plates, standards, buffers, and other reagents to run the machine adds up. This was a major consideration.
(b). Finding very specific antibodies that will work at high concentrations was not easy. Traditional Abs that work in Western did not seem to be transferrable to the machine. Some worked, some did not.
Hope this helps!
We had the early version of the Protein Simple system. This was the one in which all capillaries were to be prepared manually. But I think the separation steps work the same.
Anyways, the system is good for doing 'assembly line' type work where one is using a small set of pre-characterized antibodies and cells (lysates). if you are going to be repeating the same type of detection day after day, then this system is perfect. Although, you still have to find the best conditions for the Ab and sample, if these two are the same all the time, then you can run many assays for e.g an experiment where you just change the treatment conditions of cells and look at the same protein.
We did not find it convenient enough to be able to replace Western blotting. (a). The cost of plates, standards, buffers, and other reagents to run the machine adds up. This was a major consideration.
(b). Finding very specific antibodies that will work at high concentrations was not easy. Traditional Abs that work in Western did not seem to be transferrable to the machine. Some worked, some did not.
Hope this helps!
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