How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

How could I get the P-value from the mean, SEM, and n of different groups in the t-test?

How could I get the P-value from the mean, SEM, and n of different groups in the t-test without the original data? For detail, I would like to know the P-value between BMP-2: 50ng/ml group and the...

06 February 2021 6,539 4 View

What mechanism is involved when chitosan is coated on magnetic nanoparticles?

Hello! I am currently working on coating of chitosan on magnetic Fe3O4 nanoparticles. I am wondering how this coating process happened? i.e. through what mechanism that chitosan can be coated on...

13 January 2021 5,657 5 View

The impact of different poisson's ratios when analysising field vibration?

The Poisson's ratio of soil is quite difficult for us geo-engineers to obtain. I came across a problem when I tried to determine the parameters in a FEM model. For the saturated clay in...

02 January 2021 8,971 3 View

Is it possible to deposition a large area dielectric layer(mm scale) on the wet transferred graphene?

I want to deposition a dielectric layer on top of the CVD wet transferred graphene. The purpose of the dielectric layer is to uniformly cover the graphene and enable the top-gate modification of...

14 December 2020 7,693 2 View

Is the fluorescent protein must be totally photobleached in FRAP assay?

Hi I have tried to measure the mobility of a protein of interest by the FRAP assay. The protein was fused with GFP. I overexpressed the fusion protein in Hela cells. However, when the area of the...

14 December 2020 6,567 1 View

Is there some effective transfection reagents or methods for transfecting plasmids to ExpiCHO-S cells?

Dear all, We want to transfect pCDH plasmid or other plasmids to suspension ExpiCHO-S cells and express our target proteins, and we used the transfection reagents PEI. Unfortunately, there...

26 November 2020 8,323 3 View

No title

The cell I used is 293T.I want to it can grow on the biocompatible materials,like agarose or alginate gels.But the shape of the cells is not good.In the T25 flask you will find the cell like...

26 November 2020 1,009 2 View

Again, what are the bands of extracted plasmid?

I used Trans1-T1 phage resistant chemically competent cells to extracted lentiviral gRNA expression vectors. And there were bands near 2000bp and smear on 1% argrose gel. Did the smear come from...

25 November 2020 858 2 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

How to modify micro-structure of Pure Copper which is available as a flat plate of 25 X 25 sq. cm?

Hi everyone, for a thesis project I have to modify the micro-structural properties of pure Cu flat plate of dimension 25 X 25 sq. cm . As I tried searching the available literature, I found a lot...

09 February 2021 5,090 3 View

Can I use aluminum or copper based substrate for DNA origami imaging using SEM?

As suggested in the title I want to image my DNA nanoflowers/origami using SEM. These nanoparticles are an organic-inorganic particle made out of highly packed DNA and also contain magnesium...

28 January 2021 3,343 3 View

What is the maximum voltage a piezoelectric PZT layer can handle?

I have a circular PZT layer deposited by PLD method. The dimensions are: 20um diameter and 350nm thick. It has 100nm Pt electrodes sputtered on the top and bottom. I would like to know what is the...

25 January 2021 688 5 View

What causes the temperature variation of Carbon/PAEK composite sample while heating with AC current at certain voltage in a number of cycles/one time?

It should be either decrease or increase for nth time during heat supply. Is there any possibility of irregular temperature sequences as shown in figure. In that picture the highest temperature...

12 January 2021 4,390 4 View

What to do with this Gaussian Convergence error?

Hi! I'm kind of a beginner with computational chemistry and I\m trying to study the interaction between copper ion and a graphitic carbon nitride quantum dot. the calculation converges with HF but...

02 January 2021 8,684 5 View

Why cannot get any graphene on the surface of copper foil after a CVD growth process?

Parameter: 0.3 sccm CH4 + 2 sccm H2 / 1000 C / 350 mTorr / 1-15 min Pretreatment: Electropolishing + 30 min anneal with 10 sccm H2 Tried several times but get no carbon on copper, then tried to...

01 January 2021 9,754 3 View

Is grain boundary scattering 'always' dominant over surface scattering in grained metal/semiconductor thin films?

Hi all, I was wondering if there is some theoretical insight in the relative contribution to the resistivity of grained thin metal or semiconductor films coming from bulk grain boundary...

18 December 2020 2,969 3 View

How can we remove copper from copper mine tailings, on an industrial scale?

Removing copper from copper mine tailings, to reduce environmental pollution

13 December 2020 2,037 8 View

Can I perform Zeta-potential analysis with for nanoparticles dispersed in propylene glycol using disposable capillary cell (DST1070) ?

I am conducting a zeta-potential experiment but it shows I have a poor quality result and my sample conductivity is low. My sample is copper nanoparticles dispersed in propylene glycol.

05 December 2020 3,966 9 View