We are working on recombinant protein expression in Pichia and getting expression in small scale but not getting any expression in bulk culture.

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Julian Avila

If you are using Methanol for induction try increasing the concentration, or feed the culture every 12 hours. Your large scale culture, if well aerated will loose more Methanol than the smaller scale ones.

Sanjeev Kumar Bhure

Yes we had already tried inducing every 12hrs and also tried methanol from 0.5 -2.0%... still unsuccessful in getting good expression