Do you have good experience with a certain equipment for synthesis and what are the costs?
What would be a suitable method for purification?
Are there alternatives to MS for QA?
Thanks!
I used two instruments from Gyros Protein Technology.
One is PurePep Chorus and the other one is Tribute UV-IR. I personally prefer Tribute® UV-IR because it generates less wastes. It also dissolves the amino acids before the reaction. Anyways, both are ok. The costs would depend on where you are and what you are trying to do. You can get an estimate from their website. https://www.gyrosproteintechnologies.com/
I have a suitable method for purification on my paper. You can access it here:
https://pubmed.ncbi.nlm.nih.gov/35406720/
You can use Kaiser Test to make sure peptide bonds are actually formed instead of MS, but I would not recommend it. I hope it helps.
We have experience with LibertyBlue from CEM, PS3 peptide synthesis, and shakers. They all worked well. The choice depends on your purposes and demands.
Regarding purification, I strongly recommended avoided any purification. Instead, it is worth allocating more effort to synthesis. Based on my experience, most SPPS do not need purification.
Note: I work for Teledyne ISCO.
Please consider Teledyne ISCO preparative HPLC and flash chromatography systems for peptide purifications. Although, ideally, solid phase synthesis shouldn't require purification, the synthesized peptides do require purification.
See:
https://www.teledynelabs.com/en-us/resources/Documents/Application-Notes/Flash-Peptide-Method-Development-Part-1---EGFRvii.pdf
https://www.teledynelabs.com/en-us/resources/Documents/Application-Notes/Flash-Peptide-Method-Development-Part-2---Thymosin.pdf
https://www.teledynelabs.com/en-us/resources/Documents/Application-Notes/Flash-Peptide-Method-Development-Part-3--AS48.pdf
This application note explains why pH buffering is useful for peptide method development. The mass spectrometer helped to identify peaks containing the peptide that would otherwise be lost, being resolved from the larger peak, as impurities:
https://www.teledynelabs.com/en-us/resources/Documents/Application-Notes/Preparative-Chromatography-Focused-Gradients,-pH-Control,-and-Ionizable-Compounds-2-Peptides.pdf
https://www.teledynelabs.com/en-us/resources/Documents/Application-Notes/Purification-of-Peptide-Peptoid-Hybrids.pdf
https://www.teledynelabs.com/en-us/resources/Documents/Application-Notes/Purify-lots-of-peptide-with-RediSep%C2%AE-8.pdf
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