How to make an apparatus for testing membrane permeability and selectivity?

Good afternoon everyone! I just can't find drawings or a description of the creation of an apparatus for determining the permeability and selectivity of membranes in relation to gases and gas...

14 January 2021 9,205 4 View

Fixation of whole rats organs in buffered formalin?

Dear colleagues, I would like to ask you if anyone has experience with the fixation of whole rat organs (heart, lungs, liver, kidneys, brain, testes) in 10% buffered formalin. Thank you, Roman

28 October 2020 3,175 3 View

How can you do biotynilation of antibodies in presence of gentamicin?

For some time we have used biotinylated antibodies for ELISA. Usually I use biotin N-hydroxysuccinimide ester to link biotin to antibodies (and I have used different carbodiimides for other...

28 July 2020 8,806 3 View

If you had to reference a publication which proves or at least rigorously argues that AI control problem is (un)solvable, what would you cite?

Links to such articles with some explanations would be great!

27 July 2020 7,035 6 View

What is the estimated projected price of electricity (or hydrogen) for bio-solar cells?

Hello, everyone! There are many reports about bio-based solar cells and their prospects in the scientific literature. All authors write that these devices will be low-cost. Does anyone know any...

13 July 2020 655 3 View

Can you help me?

I am looking for information as to whether some insects (or spiders?) similar in pattern of colouration (red anteriorly, blackish posteriorly, white transverse bands) to some Cleridae (Clerus,...

09 February 2020 4,416 3 View

Does somebody know about protocol of retroviruses and adeno-assosiated viruses?

Hello! I have a lot of practice with lentiviruses (shRNA, knock-in, CRISPR, etc.). And now I need to transduce human and mouse cells by retroviruses (knock-in). I recieved plasmid pQCXIP and in...

11 January 2020 1,245 3 View

Variation partitioning - Is there a all -encompassing function/package in R?

I am a bit frustrated looking for a competent function doing variation partitioning (e.g. on linear model output) it should be able to tick the following boxes A) calculate all overlaps and "pure"...

04 December 2019 9,273 3 View

Is there any problem with magnetite nanoparticles to be observed in a FESEM system?

I ask this because I had a problem with a FESEM FEI SCIOS system. According to the technician he made observations of my sample TiO2/MAGNETITE. After many observations and session of my samples...

25 November 2019 3,024 4 View

Has anyone had problems quantifying DNA using Qubit?

I use Qubit to quantify my DNA material, and I have never had quantification problems. However a few weeks ago my lab bought a new Qubit reactive which has a different color (slightly pink) in...

23 September 2019 3,943 1 View

Which CGRP I could use to treat murine cells in vitro?

Does anyone worked with CGRP on mice. I m stuck with which the product good to use. I found Sigma products are good but Sigma has a lot of CGRP products and papers didnt mention the code or cat#...

16 February 2021 8,344 2 View

Best loss function for special classification problem in neural networks ?

Hello, I've build a Conv1D model to classify items into 6 categories (from 0 to 5). (simplified example) I use therefore a CrossEntropy loss function that penalizes the errors with high...

19 January 2021 3,199 1 View

How do I determine the minimum number of observations needed per category in categorical predictor variables?

When conducting a simple linear regression analysis with one categorical predictor variable and power projected at .80, G*Power suggests a minimum sample size of n = 55. Now, assume that the...

10 January 2021 6,317 6 View

To become an Ethologist, what classes should I take freshman year of college to set me on that path?

I am currently a freshman in college aiming to become an Ethologist. I am wondering what classes I should take freshman year to set me on that path. My ultimate goal is to study big cats (lions,...

15 November 2020 5,212 15 View

LIFE in SMART CITIES: Animal data versus Human data, complementarity or not?

One observation is that in any city (those I know) you have humans, of course, but you also have many animals. Statistics of humans start with population data (for instance demographics) and...

08 November 2020 4,127 6 View

How to run a HSIAO test in STATA?

Hello friends, I am currently doing research about the determinants of financial self-sufficiency for moroccan MFIs, I have a data set of 6 individuals and 15 years. I learned that I have to run a...

01 October 2020 2,309 3 View

What is appropriate to use as my affiliation for a manuscript after finishing graduate school?

I would like to publish my graduate (M.S.) research, which is wildlife/conservation-related, and which I conducted at a U.S. university. It's been a few years since I finished graduate school. I...

14 September 2020 6,153 19 View

Are my birds safe from COVID-19, and is my family safe around my birds?

07 September 2020 4,102 17 View

Feline immunodeficiency virus (FIV) radioimmunoprecipitation assay (RIPA) protocol?

Can someone share an FIV RIPA protocol? I have FIV infected/control cat sera that I want to check for antibodies to FIV proteins. Looking for a cell transfection with...

31 August 2020 8,487 1 View

Is it possible that cat owners have higher immunity for COVID-19?

In a paper deposited in BioRxiv entitled: "Susceptibility of ferrets, cats, dogs, and different domestic animals to SARS-coronavirus-2" the team from Harbin Vet Institute intentionally infected...

04 April 2020 3,630 21 View