How to interpret multilevel model with categorical variables (fitted using brm() in R)?

I am using brm() in R to fit a multilevel model for repeated measures data. The two predictors are both categorical. One is `Task` with two levels (`LO` and `ID`), another one is `Condition` with...

10 November 2020 712 4 View

Can Scheirer Ray Hare test be used in unbalanced data and sample size <5?

Hi everyone, I have a "tricky" dataset with two factors but is unbalanced and sample size small than 5. And this data is not a normal distribution but a bimodal. So I think ANOVA is not very...

29 July 2020 2,707 1 View

Has anyone ever done research on employment for people with disabilities especially in public service?

I am currently doing my research on employment rate of people with disability in public service. I would be glad if someone has done or is doing the same topic.

19 March 2020 4,631 3 View

How to statistically analyze bad replication experiment data ?

11 March 2020 7,545 7 View

What is wrong with the plates?

I conducted a plaque assay in a 24-well plate. All 12 of the plates had the same problem shown in the attached pictures. I did a mock assay before this and it went well without any problems. Does...

25 December 2019 3,833 2 View

Reliable conjugated and unconjugated IL17-F anti-human antibody for flow cytometry?

Dear all, can someone kindly recommend a reliable fluorochrome-conjugated IL17-F anti-human antibody and also an unconjugated one please? I am studying CD3+ Lymphocytes from healthy volunteer...

19 February 2019 3,843 3 View

How to measuring extracellular ATP level?

How to measuring extracellular ATP level in tumor specimens, not the culture cell? Is there are commecial Kits available?

17 November 2018 1,053 1 View

How to analyze flow cytometry data?

I analyzed the data of my flow cytometry assay. Data of Capture-1 is about cell apoptosis induced by nanoparticles in cell culture. 1. Was the gate set correctly? 2. Why did the clusters of the...

24 April 2018 1,916 2 View

Is it possible to run SDS-PAGE with sample of Nanoparticle combined with Albumin?

I add nanoparticle and Albumin together, incubator 24 hours. I run SDS gradient gel with this combination after 10min, 70 degree denature. I got something like half way waterfall on the gel....

09 January 2018 4,804 3 View

Question about Rag2-/- mice for tumor model establishment?

Rag2-/- mice are more immunodeficient than nude mice, though both show highly activated NK. However, few literature reported human-derived tumor bearing Rag2-/- for cancer diagnostics and...

07 November 2017 4,907 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View