How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Has any research on translation investigated the phenomenon of bilingual authors who produce versions of their books in both their languages?

Can the second-language version, strictly speaking, be considered a translation of the first-language version or is it merely a somewhat looser interpretation? How do such authors approach the...

10 December 2020 5,092 25 View

How to distinguish CA1 pyramidal cell vs. interneuron using voltage-clamp?

I am doing voltage-clamp experiments in CA1 pyramidal cells (mEPSCs; AMPA-NMDA ratio; LTP; etc) from acute hippocampal slices in mice. I want to make sure I am only recording from excitatory...

29 October 2020 1,098 2 View

Increase Oleophilicity while decreasing hydrophilicity on silicon surfaces?

Hello RG community, currently I am trying to reach these goals on silicon surfaces for a microfludic project. I have used silanes like APTES in the past in order to get my si-surface hydrophobic...

22 March 2020 2,925 3 View

How could one improve the magnitude of t-statistics of a construct?

Hello All, I am not even sure if this is possible, but I have used this dataset for several publication -albeit with different constructs-and have not seen such low t-values. For example, I have a...

10 March 2020 1,164 3 View

How do I calculate the Mean and Standard Deviation for a set of items (i.e., that form a construct) in SPSS?

Hello All, I have been asked to supply the Mean and Standard Deviation for my latent constructs. Is there a straightforward way of doing it in SPSS? I am actually modeling in SmartPLS, but not...

04 March 2020 1,597 4 View

Any strategies for quantification and purification of a red pigment with no peaks in the visible region which is only soluble in water?

We have isolated a red pigment producing bacteria. We harvested the crude pigment by removing the cells by centrifugation. We tried to find the absorption maxima (lambda max) of the pigment using...

19 January 2020 1,331 2 View

How to Create Small Graphs for Interaction Effects?

Hello All, I have been able to calculate all the interaction effects for a study involving the Big Five. One of the interaction effects I would like to graph (among others) is the relationship...

30 December 2019 6,640 3 View

Can you have stop codon right before a RBS (Shine-Dalgaro) site?

I am designing a primer for a gBlock and I don't want to the poly-histag. So naturally I am adding a stop codon in my primer, but then I need a new RBS site. So my question is can I have a stop...

28 July 2019 1,675 2 View

XML haar cascade for red blood cells and/or echinocytes ?

Hello community, we currently working on an automatic algorithm for erytrocyte detection in stereo-microscopy images for cell counting. Additionally we want to determine the percentile amount of...

23 October 2018 3,262 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View