Wanted to ask my senior researchers about quality of antibodies from Anaspec

02 February 2010 0 5K Report

Hello I am thinking about buying my antibodies from Anaspec, california USA, have any of you used their antibodies, just wanted to be sure of the quality of the product that i shall be purchasing and do any of you have previous experience working with antiMOG antibodies from them or any of their products, if u have plz do drop a msg. Thank you

Badges
Science topic

Similar topics
Cell Biology
Methods

More Sanjaya Mani Dixit's questions See All

DCF_DA fluorescense -ROS detection in attached cells

Hello everyone Recently I have been working on ROS production studies using the dye DCF-DA fluorochrome. I came across idea that trypsinization also increases the ROS production in cells,...

03 April 2011 2,536 22 View

Western Blot-Bcl-2 Total Santa Cruz polyclonal antibody- ghost bands numerous

Hello everyone, I have started doing western blot for some months but then with the new antibody from Santacruz for Bcl-2 I got lots of ghost bands.... I have tried reducing d concentration of...

02 March 2011 6,311 6 View

Phosphorylation studies-how effective is using animal serum for blocking? TBST & PBST any significant difference?

I was doing a phosphorylation study of Tau using tau-1 antibody from Millipore, I am having trouble getting bands... on contacting the company they asked me to change the blocking solution to the...

02 March 2011 6,930 6 View

DNA ladder apoptosis-no ladder formation

Hello Everyone, I once tried performing the DNA ladder test as a measure for apoptosis. I isolated the DNA using a kit and also quantified it, it was ok back then even purity seemed ok. However...

01 February 2011 5,043 19 View

Needed some help with flow cytometry

Hello everyone, I just started with the flow cytometry and since I was doing it for the first time, I planned to move on with single PI staining for detection of dead cells-either late apoptosis...

10 November 2010 2,861 18 View

Markers of Oxidative damage-PCC..Protein turnover

Protein carbonyl content is the mostly employed marker for oxidative damage. Protein turnover seems to be a old parameter used for the similar pupose. Morphological damage is a good indicator but...

09 October 2010 2,845 3 View

N Acetyl cysteine and MTT--incompatible or am I only having problems

I recently tried using NAC on some cell line and wanted to check the viability of cells using MTT. I found that as soon as i add MTT to wells containing NAC it turns bluish black......some kin dof...

09 October 2010 3,936 1 View

Nuclear Staining methods--DAPI and Hoechst- Apoptosis....TUNEL......

Nuclear staining methods DAPI and Hoechst are both minor groove binders intercalating the AT regions. In case of apoptosis the cells may show ssDNA strand breaks as well as dsDNA strand breaks-...

09 October 2010 8,770 5 View

N Acetyl cysteine and MTT--incompatible or am I only having problems

I recently tried using NAC on some cell line and wanted to check the viability of cells using MTT. I found that as soon as i add MTT to wells containing NAC it turns bluish black instantly...

09 October 2010 2,174 4 View

CoQ10 insolubility problem for cell culture experiments

hello everyone, I have been working on drug screening at cellular level for protecting cells against oxidative stress. In doing so I choose CoQ10 as a positive control agent. Now that I...

09 October 2010 4,561 7 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View