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Questions related from Sanjaya Mani Dixit
Hello everyone Recently I have been working on ROS production studies using the dye DCF-DA fluorochrome. I came across idea that trypsinization also increases the ROS production in cells,...
04 April 2011 2,566 22 View
Hello everyone, I have started doing western blot for some months but then with the new antibody from Santacruz for Bcl-2 I got lots of ghost bands.... I have tried reducing d concentration of...
03 March 2011 6,335 6 View
I was doing a phosphorylation study of Tau using tau-1 antibody from Millipore, I am having trouble getting bands... on contacting the company they asked me to change the blocking solution to the...
03 March 2011 6,956 6 View
Hello Everyone, I once tried performing the DNA ladder test as a measure for apoptosis. I isolated the DNA using a kit and also quantified it, it was ok back then even purity seemed ok. However...
02 February 2011 5,074 19 View
Hello everyone, I just started with the flow cytometry and since I was doing it for the first time, I planned to move on with single PI staining for detection of dead cells-either late apoptosis...
11 November 2010 2,889 18 View
hello everyone, I have been working on drug screening at cellular level for protecting cells against oxidative stress. In doing so I choose CoQ10 as a positive control agent. Now that I...
10 October 2010 4,597 7 View
I recently tried using NAC on some cell line and wanted to check the viability of cells using MTT. I found that as soon as i add MTT to wells containing NAC it turns bluish black......some kin dof...
10 October 2010 3,963 1 View
Protein carbonyl content is the mostly employed marker for oxidative damage. Protein turnover seems to be a old parameter used for the similar pupose. Morphological damage is a good indicator but...
10 October 2010 2,872 3 View
Nuclear staining methods DAPI and Hoechst are both minor groove binders intercalating the AT regions. In case of apoptosis the cells may show ssDNA strand breaks as well as dsDNA strand breaks-...
10 October 2010 8,800 5 View
I recently tried using NAC on some cell line and wanted to check the viability of cells using MTT. I found that as soon as i add MTT to wells containing NAC it turns bluish black instantly...
10 October 2010 2,198 4 View
Hello I am thinking about buying my antibodies from Anaspec, california USA, have any of you used their antibodies, just wanted to be sure of the quality of the product that i shall be purchasing...
02 February 2010 4,849 0 View
I wish to see the effect of TNF alpha on rat oligodendrocytes in different stages, i have a recombinant human TNF alpha, since it shares 71-92% aminoacid sequence in ECD with that of rat, i should...
01 January 2010 1,406 2 View
Hello everyone, I recently started culturing the cells and that since i am new into sterile working and all these, i just wanted to practice a few basics of cell culture and started in absence of...
12 December 2009 7,041 4 View
