I want to isolate various microorganisms especially bacteria from soil. Therefore I need the protocols of all the available growth media.
For general isolation, you can culture the microbes after serial dilution of the soil on Nutrient Agar plates. Than you can separate each of them by further inoculating the microbes on the plates
Salam,
I suggest you to use the R2A media. It's suitable for the isolation of various taxa from soil. Another trick would be to dilute it at 1/10, in order to get a better recovery since soil microflora grows better in low nutrient media.
Cheers :)
I suppose that Farhan and Hadj forgot, that soil microbial concsortia contain also another microorganisms than bacteria. So for isolation of yeast and molde you can use malth extract broth. Pleasde have a look at
http://www.sigmaaldrich.com/analytical-chromatography/microbiology/microbiology-products.html?TablePage=17996747
There you can find various culture media for soil microorganbisms.
Best rehgards
Vit
As stated by Farhan the culture base technique for isolation of different species of bacteria is done on a simple medium like nutrient agar and further subculturing of various colony will be made for characterization and identification
Vit Mateju gave a wonderful concise answer. Please, use the recommendation.
I once used the same soil 10% (w/v) in agar and got a different community than using diluted LB or NA.
Dear Aqleem,
It is very difficult to select a medium for isolate general microorganisms from soil. As the colleagues said above, the media LB, NA and R2A are good options to obtain bacteria... For fungal isolates, the media PDA and Yeast-Malt extract are also good choices. Indeed, for bacterial isolation add some amount of fungicides, for example cyclohexamide or nistatin... For fungal isolation, add an antibiotic such as chloramphenicol...
But, first of all!! Take care about the microbial isolates obtained!! Soil harbors several pathogenic fungi and bacteria. We already had obtained some isolates of Bacillus anthracis and Cryptococcurs neoformans from directly soil isolation. So, before the identification of microbes, use the adequate safety apparatus in your laboratory.
Best regards,
Paulo Ivan
Dear Juana Munoz-Ucros,
Do you have a reference for the "soil medium"? Some groups in Brazil had studied this strategy and there are some publications in portuguese language.
Best regards,
Paulo Ivan
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