I work on Vitek 2 Compact System for identification of bacterial isolates and PCR technique using 16S rRNA gene ... but the results are mismatched... can any one tell me why
It makes sense if you see the differences in results. 16s is not always an excellent technique for the identification at species level. And the results can differ a lot on the basis of sample prep and sequence data processing. There are been several studies which compared both methods and found vitek outperforms 16s sequencing. Find attachment
Identification is always dependent on the quality of the data base you use.
GenBank is an open data base. Everybody can send his data to Genbank. There´s no quality control. So, you also might get some trashy results.
On the other hand commercial data bases like VITEK are quality controled but they probably contain only reference strains and patients´ isolates. These data bases are not really suitable for environmental isolates.
Dear Dirk Schmidt:
Our isolates from humans not from environment
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