Visualization of GFP expression in transformed plant tissues?

More Prem Kumar G's questions See All

Gene editing using Cas9 RNP?

Hi everyone, Im trying targeted gene editing in citrus protoplast through PEG-mediated transformation. Im using sgRNA+Cas9 RNP complex for DNA free genome editing. But im not successful in...

06 July 2019 1,471 1 View

How to design and insert my target sequence into sgRNA scaffold?

Dear All, I want to design sgRNA target sequence and insert into scaffold portion already present in pRGEB31 binary vector. Any experimental protocol for that? I want to knock out a disease...

05 June 2018 4,918 2 View

Anyone knows in vitro digestion of DNA with Cas9?

I am going to check my gRNA efficiency by in vitro digestion of DNA with Cas9. I read the protocol from NEB website but have some doubts regarding that. I want to know whether i should do in vitro...

05 June 2018 9,932 0 View

Standard protocol for plant tissue preparation to visualize GFP?

Dear Researchers, I am doing transformation in cotton plant. Now I want to check GFP expression in transformed tissue. Can anyone suggest a standard protocol for preparing tissue sample to...

10 November 2016 1,417 5 View

Is vacuum infiltration really effective for agrobacterium mediated transformation in seeds of recalcitrant plants?

Dear Researchers, Im trying vacuum infiltration for seed transformation of a recalcitrant plant. I tried different time durations of vacuum infiltration (1 min to 10 min), culture density...

08 September 2016 6,839 3 View

Antioxidant enzymes in cotton seedlings?

Dear Researchers, Is it possible for cotton seedlings (10 days old) to produce antioxidant enzymes in cotyledons and other parts under short duration (6h,12h and 24h) of salt and drought...

06 July 2016 4,977 3 View

Any suggestion regarding protein sample preparation for 2D gel electrophoresis?

Hi Researchers, Currently I am doing 2DGE to identify differential protein expression (unknown) in plant tissues under various abiotic stress conditions. I am following phenol extraction...

05 June 2016 407 4 View

Protein expression in cotton seedlings under abiotic stress?

Dear all, I have planned to study differential expression of proteins through 2-D electrophoresis (not any target protein) in 7 days old cotton seedlings under different abiotic stress (NaCl,...

03 April 2016 8,239 7 View

Does bubble formation affect separation of proteins in 2D electrophoresis?

Dear Researchers, I was preparing to run 2nd dimension (SDS PAGE) after finishing 1st dimension (isoelectric focusing). After finishing everything perfect, i was sealing top of the IPG strip...

03 April 2016 557 13 View

Good protocol for silver staining?

Hi researchers, for the first time i am going to do silver staining of SDS PAGE. can anyone suggest me a good protocol for doing that? Thanks in advance.

02 March 2016 1,980 6 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Dr Siva Govindarajan

Dear Prem refer this article

Sandeep Kumar Tata

using a inverted confocal laser microscope. Please check the paper-Heterologous expression of chloroplast-localized geranylgeranyl pyrophosphate synthase.

Thanks

Yuan-Yeu Yau

Hi Prem,

A review paper for you, "Fluorescent Proteins in Transgenic Plants". Hope it is helpful for you.