I am planning long-term studies of tissue remodelling in vitro using light-sheet fluorescence microscopy. I am aware of confocal reflectance microscopy and second harmonic generation microscopy for imaging collagenous extracellular matrix without labelling, but I have seen only "home-made" systems for light-sheet reflectance microscopy. I would like to avoid immuno-labelling collagen, as this would not show collagen formed since the last antibody incubation.

Does anyone have experience in such applications using light-sheet? Would collagen's autofluorescence give a reliable enough signal? I see people are often looking for ways to eliminate this signal, so it seems promising. The quality of imaging doesn't have to be as good as SHG, as that will come later in the project.